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Ginseng has been used as a traditional medicine with various therapeutic effects. However, it is still unknown which component of this plant is effective at promoting wound healing. Recently, ginsenoside Rb₂ has been reported to improve wound healing. In this study, to investigate the reported wound healing effect of the ginsenoside Rb₂, cell morphology arid protein factors involved in epidermal formation were evaluated by immunochemical and immunoblotting analysis.Rb₂ stimulated epidermal cell proliferation, and the cell showed a 1.5-fold increase in thymidine uptake compared to the control (p<0.05, H=3). Furtheremore, Rb₂ was found to stimulate epidermis formation in a dose-dependent manner in raft culture, and to dose dependently enhance the expressions of protein factors related to cell proliferation, namely, epidermal growth factor and its receptor, fibronectin and its receptor, keratin 5/14, arid collage- nase 1 (p<0.05, H=3-9). It is believed that ginsenoside Rb₂ enhances epidermal cell proliferation by upregulating the expressions of these proliferation-related factors.
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